Fig. 2

Cell fate after PTIP inactivation. A Hela Tet-on cells were treated with PTIP RNAi for 18 h and then subjected to live cell imaging for 20 h. 50 cells were counted for cell fate. Blue and green lines indicate interphase, and red lines indicate M phase. Type 1: cells survived after 20 h. Type 2: cells died in interphase. Type 3: cells died during mitosis. B HeLa Tet-on H2B-mCherry cells were transfected with PTIP siRNA oligo for 24 h and H2B-mCherrry images of live cells were taken for additional 20 h. Movies of about a hundred mitotic cells were analyzed for their fates in two independent experiments. P-value was calculated by student t-test, paired, two-tailed