Fig. 5
From: The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis
Localization of PTIP near centrosomes. A Cell cycle-specific localization. YFP-PTIP was induced by doxycycline from HeLa Tet-on stable cell line and its localization was examined by immunofluorescence using GFP antibody. Microtubules were shown by β-tubulin antibody and DNA by Hoechst 33342. Size bar, 5 µm. B Domain structures of PTIP full-length or truncations were illustrated. C Domain-specific localization. GFP-PTIP truncations are expressed in HeLa Tet-on cells and their localization in M-phase or interphase cells were examined by immunofluorescence using GFP antibody. DNA was shown by Hoechst 33342 staining. Size bar, 10 µm