Polo-like kinase 4: the odd one out of the family
© Sillibourne and Bornens; licensee BioMed Central Ltd. 2010
Received: 27 August 2010
Accepted: 29 September 2010
Published: 29 September 2010
Polo-like kinase 4 (PLK4) is a unique member of the Polo-like family of kinases that shares little homology with its siblings and has an essential role in centriole duplication. The turn-over of this kinase must be strictly controlled to prevent centriole amplification. This is achieved, in part, by an autoregulatory mechanism, whereby PLK4 autophosphorylates residues in a PEST sequence located carboxy-terminal to its catalytic domain. Phosphorylated PLK4 is subsequently recognized by the SCF complex, ubiquitinylated and targeted to the proteasome for degradation. Recent data have also shown that active PLK4 is restricted to the centrosome, a mechanism that could serve to prevent aberrant centriole assembly elsewhere in the cell. While significant advances have been made in understanding how PLK4 is regulated it is certain that additional regulatory mechanisms exist to safeguard the fidelity of centriole duplication. Here, we overview past and present data discussing the regulation and functions of PLK4.
The structure of PLK4
PLK4 was initially identified in the mouse as a kinase sharing homology with Drosophila Polo kinase, S. cerevisiae CDC5 and murine Snk (it was subsequently named as S nk a kin k inase (SAK) due to its homology with the latter) . Recent evolutionary studies have shed light onto the origins of PLK4/SAK, which appears to have arisen through gene duplication and subsequent subfunctionalization [2, 3]. Homologues of PLK4 are present in most opisthokonts (organisms that have a single posterior flagellum), with at least one exception, the nematode C. elegans. This organism has no direct homolog of PLK4 although the kinase zyg-1 has been proposed to be a functional equivalent because it is essential for centriole duplication in the worm . Interestingly, zyg-1 shares closer homology to the centrosomal kinases NIMA and MPS1 than to C. elegans Polo-like kinases (PLK1-3) strongly suggesting that it did not arise through duplication of the PLK gene .
PLK4 phosphorylation motifs
Leung et al. (2007)
I, L and V unfavoured
Charged or P
Johnson et al. (2007)
Sillibourne et al. (2010)
Aliphatic, hydrophobic or basic (small to medium)
Large residues unfavoured
Aliphatic (charged residues unfavoured)
Aromatic or aliphatic (large)
All members of the family of Polo-like kinases possess a characteristic Polo-box, a conserved 64 amino acid motif located at the carboxyl terminus of the protein, which not only dictates the substrate specificity of the kinase, but also regulates its function [12, 13]. PLK1, 2 and 3 possess two Polo-box domains at their C-terminus, while PLK4 only has one . In place of a second Polo-box, PLK4 possesses a larger crypto Polo-box domain that has weaker homology with the Polo-box domain [14, 15]. The fact that PLK4 only possesses a single Polo-box has important implications for its regulation and substrate repertoire. PLKs 1 to 3 bind to proteins that have previously been phosphorylated via their tandem Polo-boxes, which form intramolecular heterodimers and recognize the sequence Ser-pSer/pThr-Pro-X . Polo-box dimerization and binding to the phospho-motif is thought to regulate the activity of the kinase by inducing a change in its conformation, allowing the catalytic domain to have access to its substrate . Because the Polo-box and crypto Polo-box of PLK4 do not form an intramolecular heterodimer, it has been suggested that PLK4 is not subject to the same form of regulation [14, 15]. The PLK4 Polo-box does, however, homodimerize in an intermolecular manner and this may be involved in regulating PLK4 kinase activity . The Polo-boxes of PLK1-3 are also important for targeting the kinases to particular subcellular sites and in this respect the Polo-box and crypto Polo-box of PLK4 serve a similar function. Both are independently able to localize to centrosome, when expressed in fusion with EGFP , and only when both are removed from PLK4 does it fail to localize to centrosome and its function is suppressed [14, 16]. This suggests that the Polo- and crypto Polo-boxes of PLK4 are protein-protein interaction domains responsible for targeting the kinase to the centrosome although the identities of their binding partners remain to be discovered. The ability of the Polo-box and crypto Polo-box domains of PLK4 to bind to the centrosome could also be explained by the fact that both are able to self-associate with other domains within the kinase .
A further difference between PLK4 and the other PLKs is that it possesses a large central domain, which is conserved through evolution, although the function of this domain remains unknown .
PLK4 also possesses three PEST sequences, domains rich in proline (P), aspartate (D), glutamate (E), serine (S) and threonine (T) residues, which govern protein stability [1, 17]. The first PEST sequence is conserved, being present in many species including, H. sapiens, M. musculus, D. melanogaster, D. rerio and X. laevis [18, 19]. The function of these sequences in regulating the turn-over of PLK4 will be discussed in more detail later.
Functions of PLK4
Studies carried out in knockout mice have demonstrated that PLK4 is essential for postgastrulative embryonic development and is required for mitotic progression . PLK4-/- embryos arrest at stage E7.5 with increased numbers of apoptotic and late mitotic cells , while PLK4+/- embryos develop normally but have an increased incidence of spontaneous liver and lung cancers . Partial hepatectomy experiments on PLK4+/- mice identified a defect in mitotic entry and exit, with cyclin B1 accumulation being delayed and prolonged for longer than normal . Inspection of dividing hepatocytes from these mice showed that nearly one third had tripolar or tetrapolar spindles, which consequently led to the formation of disorganized liver tissue and an increased incidence of tumors . Embryonic fibroblasts derived from PLK4+/- mice have supernumerary centrosomes, frequently undergo aberrant chromosome segregation and have a higher level of aneuploidy than wild-type mice .
At present, two mitotic PLK4 substrates have been identified: the phosphatase CDC25C  and the RhoA guanine exchange factor (GEF), Ect2 . CDC25 was selected as a candidate PLK4 substrate based on the fact that this phosphatase is phosphorylated by PLK1 and PLK3 (raising the possibility that it is a common PLK substrate) and both CDC25C and PLK4 localize to the centrosome. Ect2 also seems to be a common PLK substrate, as it is phosphorylated by both PLK1  and PLK4 . Ect2 is a guanine exchange factor for the small GTPase RhoA and is required to activate it during cytokinesis to ensure correct positioning of the cleavage furrow . Association of Ect2 to the central spindle is dependent upon PLK4 activity, as it fails to localize correctly in PLK4+/- MEFs. These cells frequently undergo cytokinetic failure because of the lack of Ect2 at the central spindle and insufficient RhoA activity .
PLK4 and centriole duplication
The centrosome duplicates once per cell cycle  and PLK4 plays an essential role in this process [16, 27]. Over-expression of PLK4 in somatic cells results in the excessive formation of centrioles , the core structures of the centrosome, and in Drosophila oocytes the de novo formation of centrioles . Conversely, depletion of PLK4 by RNAi prevents centriole duplication , causing mitotic defects and in some cell lines it can induce apoptosis .
Immunoelectron microscopy has shown that myc-tagged PLK4 localizes to the outer wall of centrioles and seems to be enriched at the proximal ends . This localization is consistent with PLK4's role in centriole duplication because it is next the site of procentriole formation. However, PLK4 has also been observed, by immunofluorescent microscopy, at the distal end of the mother centriole close to the sub-distal and distal appendages . The exact function of PLK4 at the distal end of the mother centriole remains to be elucidated, but it may be involved in centriole maturation including, centriole elongation and/or appendage assembly.
PLK4 abundance and activity during the cell cycle
PLK4 abundance must be tightly controlled to ensure that centriole duplication goes according to plan, as either too little or too much of the kinase can have a deleterious effect upon the fidelity of centriole duplication. Too much PLK4, as demonstrated by over-expression of the kinase, overrides the centriole licensing mechanism and results in centriole amplification with multiple procentrioles forming around each parental centriole [16, 27]. An insufficient amount of PLK4 may also give rise to the formation of abnormal centrioles and microtubule-based structures. In HCT116 cells microtubule-based γ-tubulin-containing structures lacking key centriolar components such as SAS-4/CPAP, SAS-6, and Cep135 have been observed . These structures are commonly formed of microtubule bundles and some resemble centrioles, but lack a large number of centrosomal proteins and are unable to nucleate microtubules. Importantly, the incidence of these structures is reduced upon expression of PLK4 suggesting that there formation is due to insufficient kinase activity. Supporting these data, embryonic fibroblasts derived from PLK4 heterozygous mice exhibit an increased incidence of supernumerary centrosomes, which may, in fact, reflect the formation of abnormal microtubule-based structures .
The regulation of PLK4 stability
Upon binding to the phosphorylated degron motif the SCF ubiquitinates PLK4 and it is targeted to the proteasome for destruction [18, 19]. Two different approaches were employed to demonstrate a specific role for the SCFslimb/β-TrCP complex in regulating the turnover of PLK4. The first involved disrupting the SCFslimb/β-TrCP complex by siRNA-mediated depletion of either the cullin or slimb/β-TrCP subunits. The second approach involved mutation of the serine and threonine residues within the degron motif of PLK4 to alanine, to prevent phosphorylation and generate a stabilized form of the kinase that was no longer recognized by the SCFslimb/β-TrCP complex. Both of these approaches resulted in elevated levels of centriole amplification compared to control siRNA depletions or over-expression of the wild-type form PLK4. These results led to the proposal that the increased incidence of centriole amplification is directly attributable to the higher expression level of PLK4 [18, 19]. Mutation of the degron motif to prevent phosphorylation clearly stabilizes PLK4, but fluorescence intensity measurements have shown that there is no difference in the amount of over-expressed PLK4 at the centrosomes of wild-type or degron-mutated PLK4-transfected cells exhibiting centriole amplification. Furthermore, mutation of the degron motif appears to promote an increase in the amount of active PLK4 because a greater proportion of the kinase is S305 autophosphorylated . These results suggest that the higher incidence of centriole amplification observed in cells expressing degron-mutated PLK4, compared to wild-type, is due to an increase in the amount of active kinase.
At present the kinase responsible for phosphorylating the degron motif is unknown but there is mounting evidence indicating that autophosphorylation plays a role in regulating the stability of the kinase. A link between PLK4 autophosphorylation and kinase stability was first established when it was observed that mutation of the kinase domain, to render the kinase catalytically inactive, resulted in increased expression of the kinase compared to wild-type . A 23 amino acid region beyond the catalytic domain in mouse PLK4, which encompasses the degron motif, is heavily autophosphorylated and the deletion of this entire region vastly increases its stability and ability to trigger centriole amplification . Similarly, mutation of the serine and threonine residues to alanine in this region stabilizes the kinase and augments its ability to amplify centriole number . A more recent paper has elegantly shown that PLK4 autophosphorylation occurs in trans, where the molecules in the dimer phosphorylate each other, and confirmed that autophosphorylation is necessary to target the kinase for degradation . This finding also explains why inducible cell lines stably transfected with catalytically inactive PLK4 are able to trigger centriole amplification upon induction of gene expression. Catalytically inactive PLK4 dimerizes with the endogenous kinase, but is unable to phosphorylate it, which effectively protects the endogenous kinase from degradation because the SCFβ-TrCP complex cannot bind to it. As the endogenous kinase in no longer under the control of this ubiquitin-mediated degradation pathway centriole amplification ensues .
Where SCF-mediated ubiquitination takes place in the cell has yet to be determined conclusively. Components of the SCF including Skp1 and culllin 1 have been found localize to the centrosome throughout the cell cycle [62, 63]. Cullin 1 seems to be enriched on the mother centriole , where there is more PLK4 and centriole duplication is probably first initiated , suggesting that there may be a greater demand for SCF activity at this site to prevent centriole amplification.
While it is clear that the SCFβ-TrCP ubiquitin ligase complex has an important role in targeting PLK4 to the proteasome for degradation, it is not the only factor controlling the kinase's turn-over and stability. Drosophila PLK4 SCFslimb-binding mutants that can no longer be phosphorylated on the degron motif are still subject to degradation in G2 phase of the cell cycle . The introduction of similar mutations in mouse PLK4 still results in degradation of the kinase. It is possible that in the absence of SCFβ-TrCP activity the APC/C ubiquitin ligase complex may take over, as this ubiquitin ligase has been proposed to be involved in regulating PLK4 degradation before .
Alternative forms of regulation
The stability of PLK4 may be governed by alternative mechanisms such as the phosphorylation-dependent stabilization of PLK4 by other kinases and there are data to suggest this is the case. Yamashita et al demonstrated that phosphorylation of a tyrosine residue in the N-terminus of PLK4 by the kinase Tec increased the stability of PLK4 and promoted PLK4 autophosphorylation . The Tec-dependent increase in PLK4 stability is interesting because it suggests that phosphorylation by other kinases may play a role in governing its turn-over. Such a mechanism might be at work at centrosome and it could result in the local stabilization of PLK4 at this site. In support of this it has been shown that S305 autophosphorylated PLK4 at centrosomes exhibits a similar shift in mobility as Tec phosphorylated PLK4 .
Another possible regulatory mechanism could be proposed from work carried out on the C. elegans centrosomal kinase, zyg-1. In a screen for suppressors of the zyg-1(it25) temperature-sensitive mutant allele a number of candidate genes were identified including s uppressor of zy g-1 20 (szy-20) [67, 68]. This gene encodes an RNA-binding protein that localizes to the centrosome and appears to negatively regulate the abundance of zyg-1 . Szy-20 is a conserved and it will interesting to see if the vertebrate homologues of this protein are involved in controlling PLK4 abundance at centrosomes.
Transcriptional control of PLK4
While much attention has been focused on proteasome-mediated degradation of PLK4 it is important not to overlook the fact that the PLK4 gene is transcribed in a cell cycle-dependent manner. PLK4 transcript levels are undetectable in G0, low in G1 and progressively increase through S and G2 to reach a maximum in mitosis . It seems that PLK4 protein levels mirror those of PLK4 mRNA suggesting that gene transcription has a significant impact on controlling the overall expression level of PLK4. At present, little is known about the transcription factors controlling expression of the PLK4 gene. One report has shown that expression of the human PLK4 gene can be suppressed by the tumour suppressor p53 and this is dependent upon the activity of histone deacetylases (HDACs) .
PLK1, PLK2 and PLK4 act in concert to control the licensing and duplication of centrioles and centrosome maturation. PLK4 represents a separate branch of the PLK family because it shares little homology with its other members as a result of rapid divergence through evolution. Its function in controlling centriole/basal body duplication is a result of sub-functionalization after duplication of the PLK gene. Before the innovation of PLK4, basal body duplication was probably under the control of a single PLK, although there are some ciliated species that do not have a PLK gene.
PLK4's role in centriole duplication is essential yet many questions remain to be answered. At present, no centriolar PLK4 substrates have been identified although one possible substrate is SAS-6, as work in C. elegans has shown that this protein is phosphorylated by zyg-1. The identification of PLK4 substrates should help to delineate the centriole duplication pathway and determine whether the kinase acts just once, to initiate duplication, or at multiple stages during the duplication process. It will also be important to identify the protein responsible for anchoring PLK4 to the centrosome via its crypto Polo and Polo-box domains. As PLK4 localizes to the proximal ends of and along the walls of centrioles it seems likely that PLK4 will interact with multiple proteins at the centrosome.
Determining how SCFβ-TrCP ubiquitin ligase-mediated degradation of PLK4 is coordinated and influenced by other factors during the cell cycle is crucial to understand how PLK4 levels are maintained within a certain threshold during the cell cycle. Clearly, if the threshold is crossed and PLK4 levels rise above normal the consequences can be catastrophic, particularly during centriole duplication because it overrides the licensing mechanism and multiple procentrioles form at each parental centriole.
The recent discovery that PLK4 is involved in cytokinetic exit broadens the role of this kinase beyond centriole duplication and demonstrates that the kinase has multiple functions in the cell. Several lines of evidence support a role for PLK4 in mitotic progression, including the identification of mitotic substrates such as CDC25C and Ect2, the delayed entry of PLK4+/- dividing hepatocytes into mitosis coupled with persistently elevated levels of cyclin B1 and the fact that active PLK4 levels reach a maximum during mitosis. This suggests PLK4 is linked to cell cycle regulators, but also raises the possibility that the kinase is involved in regulating centrosome maturation, such as procentriole elongation, which is completed in early mitosis, or the transformation of the daughter centriole into a mother centriole (appendage formation). It is clear that much work remains to be done before we fully understand the functions of this kinase in the cell.
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