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Table 2 Plasmids used in this study.

From: The loop-less tmCdc34 E2 mutant defective in polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2

Plasmid Description Reference
pYL150 2μm, LEU2, Amp r , GAL10 promoter +CDC34 [17]
pYL19 pYL150 with S97D mutation in CDC34 [17]
pYL27 pYL150 with 12-residue deletion of G-103 to T-114 in CDC34 [17]
pYL29 pYL150 with 12-residue deletion of G-103 to T-114 and S73K and S97D mutations in CDC34 [17]
pUBP14 ADH1 promoter and terminator elements + UBP14 in pVT102-U [38]
pUBP14-Ala354 ADH1 promoter and terminator elements +catalytically inactive UBP14(C354A) mutant in pVT102-U [38]