- Open Access
Translational control of cyclins
Cell Division volume 6, Article number: 5 (2011)
Regulation of cyclin levels is important for many cell cycle-related processes and can occur at several different steps of gene expression. Translational regulation of cyclins, which occurs by a variety of regulatory mechanisms, permits a prompt response to signal transduction pathways induced by environmental stimuli. This review will summarize translational control of cyclins and its influence on cell cycle progression.
The cell cycle is a fundamental and ordered process in which DNA is replicated and homologous chromosomes are segregated and equally distributed to daughter cells. The rate of protein synthesis oscillates during the cell cycle, indicating the importance of translational control for cell cycle progression . Moreover, translational control allows rapid and reversible alterations of protein levels in response to various physiological and pathological conditions . Therefore, it is important for us to have a comprehensive understanding of cell cycle-dependent translation control.
Prior to DNA synthesis, the cell cycle phase termed G1 is a period of cell growth and characterized by a high level of both protein synthesis and metabolic rate. During G1, cells also need to ensure their competency to undergo mitosis . After passing through the G1/S checkpoint, cells enter S phase for DNA replication. However, most mammalian cells pause during G1 and enter a quiescent stage termed G0; certain cell types (e.g. neurons and muscle cells) may remain at this stage and undergo differentiation. Global protein synthesis is largely down-regulated in G0, but a subset of mRNAs is specifically translated to ensure cell survival . At G2/M phase, ~60-80% of cap-dependent translation is inhibited whereas alternative mechanisms of translation may be activated for expression of certain mitotic factors .
Many key regulatory factors are expressed and activated at very specific points during the cell cycle. For example, the activity of cyclin-dependent kinases (Cdks) oscillates throughout the cell cycle and is essentially modulated by associated cyclins. The expression level of cyclins is primarily regulated by transcription of cyclin genes and turnover of cyclin proteins [4, 5]. Over the past two decades, however, translation has also emerged as a key point at which the levels of cell cycle regulators are modulated. In this review, we discuss current knowledge on the translational control of cyclins.
Translation is essentially divided into three stages: initiation, elongation and termination. Eukaryotic translational control mainly occurs at the initiation step, which engages a large number of eukaryotic translation initiation factors (eIFs) and the ribosomal subunits [6, 7]. In canonical cap-dependent translation initiation, the eIF4F complex, which is composed of the cap-binding protein eIF4E and two other initiation factors, eIF4G and eIF4A, binds to the 5'-end cap structure of mRNAs. eIF4G acts as a scaffold protein to mediate the interaction between eIF4E at the 5' end of mRNA and the poly(A) binding protein (PABP) that binds to the 3' poly(A) tail, thus circularizing the mRNA. Subsequently, the 43 S pre-initiation complex containing the 40 S ribosome, the eIF2-GTP-Met-tRNAi ternary complex and several initiation factors, joins eIF4F-bound mRNA and scans the mRNA for the AUG initiation codon. Some mRNAs harboring secondary structure or with a high GC content in the 5' untranslated region (UTR) may require additional trans-acting factors for ribosome scanning . After initiation codon recognition, the 60 S ribosomal subunit joins to form the 80 S initiation complex.
Cellular signaling pathways that affect translation and regulate cell cycle progression
During the cell cycle, several cellular signaling pathways are induced and regulate cell cycle progression via control of the translation of cell cycle factors; the most important are the Akt/mammalian target of rapamycin (mTOR) and Ras/mitogen-activated protein kinase (MAPK) pathways  (Figure 1). A number of growth stimulating factors such as growth hormones, cytokines and nutrients initially activate the phosphoinositide 3-kinase (PI3K) and Akt kinase. PI3K/Akt signaling suppresses the activity of the Rheb GTPase activating complex (TSC1/TSC2) and thereby increases the level of GTP-bound Rheb, which in turn induces mTOR signaling . mTOR signaling can target to several translation factors or regulators (see below for the detail). Activation of mTOR up-regulates the translation of key factors required for cell cycle progression from G1 to S phase, including specific G1/S cyclins, and thus promotes cell proliferation . Inhibition of mTOR results in G1 arrest in some mammalian cells . Moreover, mTOR signaling also promotes completion of the first mitotic division in sea urchin embryos by promoting cyclin B translation . On the other hand, growth-inhibiting signals can activate the AMP-activated protein kinase (AMPK) that directly phosphorylates and activates TSC1/TSC2 and therefore causes mTOR inhibition .
Growth factors or cytokines can activate MAPKs such as p38 and extracellular signal-regulated kinase (ERK). These MAPKs subsequently activate distinct families of MAPK-activated protein kinases, such as Mnk1/2, that in turn directly phosphorylate certain translation factors. For example, phosphorylation of eIF4E by Mnk1/2 can facilitate cell proliferation and has been implicated in cancer development . It is also conceivable that all above signaling pathways may be integrated to form networks for cell cycle control via common translation factors (also see below).
Translation factors are regulated by cellular signaling pathways
Cellular signaling pathways primarily target to translation initiation factors or regulatory factors. Formation/disruption of the eIF4F complex constitutes a major mechanism for controlling cap-dependent translation. The eIF4E-binding proteins (4E-BPs) compete with eIF4G for the same binding site on eIF4E and therefore can prevent eIF4F assembly . The phosphorylation status of 4E-BPs regulates their reversible binding to eIF4E. Hypophosphorylated 4E-BPs bind strongly to eIF4E, whereas the hyperphosphorylation of 4E-BPs (induced by various cellular stresses) prevents binding. The mTOR signaling pathway targets several translation factors, including 4E-BPs, and mTOR-induced phosphorylation of 4E-BPs can contribute to the increase of cap-dependent translation activity during G1 and thus promotes G1 to S progression  (Figure 1). eIF4E itself can also be phosphorylated by several different signaling kinases. The level of eIF4E phosphorylation increases in G1 and S phases and is reduced in M phase . Although the impact of eIF4E phosphorylation on translation efficiency has been a matter of debate , its phosphorylation via Mnk may result in increased translation of factors involved in cell cycle progression and also has been implicated in cell transformation  (Figure 1). Moreover, eIF4E may promote the nuclear export of cyclin D1 mRNA and thus facilitates cell cycle progression . Therefore, Mnk-mediated phosphorylation of eIF4E supports cell proliferation at least in part by promoting synthesis of specific proteins.
The eIF2-GTP/Met/tRNAi ternary complex is another important signaling target at the translation initiation step. Phosphorylation of the eIF2α subunit prevents the recycling of inactive eIF2-GDP to active eIF2-GTP and thus reduces the level of the active ternary complex . A number of protein kinases have been reported to phosphorylate eIF2α, mostly under cell stress conditions. For example, the PKR-like endoplasmic reticulum kinase (PERK) phosphorylates eIF2α in response to endoplasmic reticulum (ER) stress induced by unfolded protein response . In the fission yeast, Schizosaccharomyces pombe, that ultraviolet irradiation in G1 phase activates the Gcn2 kinase, which in turn suppresses general translation by phosphorylation of eIF2α and, accordingly, delays S phase entry  (Figure 1).
Translation may also be regulated at the elongation step. The elongation factor kinase (eEF2K), also known as a Ca+2/calmodulin-dependent kinase, phosphorylates and inactivates eEF2 (Figure 1). eEF2K activity can be modulated by the mTOR signaling pathway or AMPK [24, 25]. For example, mTOR complex 1 (mTORC1) inactivates eEF2K by activating the mitotic Cdk1/cyclin B kinase, which may ensure an adequate level of translation during mitosis .
Mechanisms of translational control during cell cycle
Cellular signaling pathways induced by changes in environmental conditions often regulate the efficiency of global translation by modulating the activity of essential translation factors. However, some mRNAs undergo specific regulation via cis-acting sequences in their 5' or 3' UTRs, such as upstream open reading frames (uORFs) or internal ribosome entry sites (IRESs) in the 5' UTR or binding sites for specific RNA binding proteins or microRNAs (miRNAs) in the 3' UTR  (Figure 2). Moreover, a single gene may generate multiple mRNA isoforms with different translation efficiency via use of alternative transcription start sites or polyadenylation sites or alternative splicing. Therefore, translation control is also linked to other steps of post-transcriptional gene expression.
uORF in the 5' UTR
Short ORFs that are located upstream of or sometimes overlap with the main protein-coding region often disrupt translation of the downstream ORF(s) . An early survey revealed the presence of conserved upstream AUGs in ~20-30% of mammalian genes . A recent study showed that uORFs indeed influences the expression of thousands of mammalian genes . The best-studied example of uORF-mediated translational control is that of the yeast transcription factor GCN4 mRNA , which contains four uORFs in its 5' UTR. Translation initiates at uORF1 and then reinitiates at downstream uORFs, leading to inefficient translation of GCN4. During amino acid starvation, the reduced level of functional eIF2 ternary complex retards reinitiation at downstream uORFs, and thus skips these inhibitory uORFs and enhances GCN4 translation. uORFs have been identified in many genes encoding cell growth/survival factors, including bcl-2, c-mos, mdm2, and her-2, and also in genes implicated in the biosynthesis of polyamines, which are important for mitotic spindle formation and chromatin condensation [29, 30]. For example, uORF-mediated translation control of an mdm2 mRNA isoform accounts for mdm2 overexpression in some tumors . Moreover, a recent report indicates that disruption of translation initiation within an uORF of the transcription factor C/EBPβ transcript delays S-phase entry . Therefore, the observed high abundance of uORFs in mammalian transcripts suggests a role for uORF-regulated translation in physiology and pathogenesis.
Secondary structures in the 5' UTR
An early estimation has indicated that ~10% of mRNAs contain atypically long 5' UTRs, and most of these mRNAs encode proteins implicated in cell growth, death or proliferation . Experimental evidence has indicated that longer 5' UTRs account for inefficient translation of certain members or mRNA isoforms of the transforming growth factor β (TGF-β, the tumor suppressor BRCA1 and oncoprotein mdm2 [34–36]. A recent study indicated that two-thirds of human mRNAs harbor a moderate degree of secondary structure in their 5' UTR . Such structures could conceivably impede ribosome scanning or block access of initiation factors to the mRNA and thus reduce translation efficiency. Translation of long or structured 5' UTR-containing mRNAs may require RNA helicases to disrupt RNA duplexes or high-order ribonucleoprotein structures during translation initiation . In fact, several DExD/H-box RNA helicases, including Ded1/DDX3, DHX29 and RNA helicase A, have been demonstrated to facilitate translation initiation of such mRNAs [37–39]. Moreover, we recently reported that translation of cyclin E1 mRNA containing a highly GC-rich 5' UTR is particularly facilitated by the RNA helicase activity of DDX3 . There are probably other RNA helicases that have potential to regulate the translation of perhaps a large number of mRNAs containing structured 5' UTRs.
IRESs are RNA structural elements in the 5' UTR that directly recruit ribosomes to the mRNA for translation initiation, thereby bypassing the requirement for the integral eIF4F complex to recognize the 5' cap of the mRNA . In addition to many viral mRNAs, IRESs have been confirmed or predicted in a number of cellular mRNA, many of which encode cell cycle regulators such as ornithine decarboxylase, c-myc, and the Cdk-like kinase p58PITSLRE . Ornithine decarboxylase is involved in polyamine biogenesis, which has an important function for mitosis. The ornithine decarboxylase mRNA undergoes cap-dependent or cap-independent translation initiation at different cell cycle stages; the latter, which is mediated by an IRES, is particularly activated at G2/M . Thus, it is likely that IRES-mediated translation initiation ensures the expression of certain cell cycle or growth factors to allow mitosis to proceed. However, how translation initiation at different types of IRESs engages different combinations of initiation and regulatory factors remains to be deciphered. Moreover, whether previously identified cellular IRESs indeed confer IRES activity has been debated and certainly requires further examination .
Cytoplasmic polyadenylation of the 3' UTR
Cytoplasmic polyadenylation was initially observed in Xenopus oocytes where it controls translation of a set of maternal mRNAs such as cyclin B1 and c-mos during oocyte maturation [45, 46]. These mRNAs contain the cytoplasmic polyadenylation element (CPE) that is located nearby the polyadenylation signal within the 3' UTR. The CPE-binding proteins (CPEBs) play a central role in regulating translation initiation of CPE-containing mRNAs. While binding to CPE, CPEB recruits the eIF4E-binding protein maskin, which excludes eIF4G, and thus inhibits translation. On the other hand, both the deadenylase PARN and the poly(A) polymerase Gld2 are also a part of the CPEB-containing complex . However, deadenylation by PARN is more efficient and/or Gld2 activity is inhibited so that the poly(A) tail of CPEB-bound mRNAs is shortened in immature oocytes. Oocyte maturation signals induce phosphorylation of CPEB by several different signaling pathways. Phosphorylated CPEB dissociates both maskin and PARN, leading to eIF4F complex formation and predominant polyadenylation, which thus allows reactivation of translation. In early oocytes lacking maskin, the eIF4E-binding protein 4E-T may substitute for maskin to suppress the ability of CPEB to promote polyadenylation . In neurons, neuroguidin acts as a functional analog of maskin to regulate the translation of CPE-containing mRNAs . Therefore, CPE-mediated cytoplasmic polyadenylation and translation plays an important role in early embryonic development and local protein expression in neurons .
RNA-binding protein-mediated translation control
Many RNA-binding proteins control translation in an mRNA-specific manner via binding to cis-regulatory elements in UTRs. The mechanisms of such translational regulation have been more extensively studied in Drosophila. For example, the Drosophila RNA-binding protein Bruno binds to Bruno response elements (BREs) within the 3' UTR of target mRNAs such as oskar and recruits the eIF4E binding protein Cup to preclude eIF4G associating with eIF4E and thereby inhibits translation of BRE-containing mRNAs . Moreover, Bruno may induce mRNA oligomerization, which prevents accessibility of ribosomes . Intriguingly, the Xenopus Bruno-like protein BrunoL1 binds to the GU/AU-rich BREs of cyclin A2 mRNA, but it promotes, rather than suppresses, translation of cyclin A2 . This observation may not be completely unexpected, however, because these translation regulatory factors may interact with different partners or have different post-translational modifications under different cellular conditions, and therefore differentially affect translation. In addition, mammalian Bruno-like/CELF proteins have been implicated in pathogenesis of the CUG-trinucleotide expansion disease myotonic dystrophy . Therefore, it will be important to elucidate the mechanisms of post-transcriptional control mediated by various RNA-binding proteins, particularly those involved in critical cellular processes or pathogenesis.
microRNA-mediated translational repression
The single-stranded miRNAs of ~21 nucleotides mediate post-transcriptional gene silencing in the cytoplasm through imperfect base pairing to the 3' UTR of various target mRNAs. miRNAs function in a multi-component complex, termed the RNA-induced silencing complex, to suppress translation and/or cause mRNA degradation . The Argonaute family proteins act as the essential effector in this complex. An estimated >1000 miRNAs are encoded by the human genome and may target ~60% of mRNAs . Some miRNAs are involved in cell cycle control by targeting critical regulatory factors, such as E2F and c-myc, cyclins, Cdks and Cdk inhibitors; misregulation of such a control may contribute to tumorigenesis . Notably, many mRNAs have binding sites for multiple miRNAs. Moreover, the activity of miRNAs can be influenced by the accessibility of their target sites in mRNAs or even modulated by RNA-binding proteins through their interaction with the target mRNAs or the miRNPs. A recent report showed that the RNA-binding protein pumilio binds to the 3' UTR of the Cdk inhibitor p27 mRNA and alters local RNA structure to enhance the accessibility of miR221 and miR-222 . Downregulation of p27 prompts quiescent cells to enter S phase. Furthermore, miRNA activity may even switch from translation repression to translation activation when proliferating cells are induced to enter quiescence [58, 59]. Therefore, miRNAs can be versatile translation regulators of the cell cycle.
Translational control of cyclins
Cyclins form a complex with respective Cdks and activate their kinase activity . Cyclins are essentially divided into the G1/S, S and G2/M classes, and function in a timely manner. Cyclin levels fluctuate during the cell cycle, which is primarily regulated by transcriptional activation and proteolytic destruction. However, accumulating genetic and biochemical evidence has indicated a role for translational control in temporal regulation of cyclin expression (Table 1).
Translational control of CLN3 by a uORF in budding yeast
The temperature-sensitive mutants cdc33 or prt1 of the yeast Saccharomyces cerevisiae show a growth defect in G1 at the non-permissive temperature [61, 62]. Because CDC33 and PRT1 encode eIF4E and an eIF3 subunit, respectively, this observation suggests the importance of translational control in G1 progression. In early G1, a burst expression of the G1 cyclin Cln3 is first observed, which subsequently induces the transcription of downstream cyclins, and is critical for progression through the G1/S boundary. Expression of the CLN3 gene is particularly impaired in the cdc33 and prt1 mutants, which may account for the G1 arrest phenotype of these two mutants . A uORF within the 5' UTR of the CLN3 mRNA regulates its expression. Inactivation of this uORF is sufficient to accelerate G1 progression . Moreover, this uORF makes the expression of CLN3 sensitive to mTOR signaling , suggesting that the level or activity of specific translation initiation factors is important for uORF-mediated translation control. Therefore, such a control may ensure CLN3 expression in prompt response to environmental changes.
Translational control of cyclin E1 by DDX3 in mammals
Mammalian DDX3, as its yeast Ded1 homolog, has been implicated in translational control [39, 64]. DDX3 may enhance general translation via its interaction with eIF3 and/or promotes the translation of specific mRNAs containing a long or structured 5' UTR by facilitating ribosome scanning during translation initiation. Our recent report showed that knockdown of DDX3 reduces cell growth rate and causes cell cycle arrest in G1 . Screening for target mRNAs of DDX3 revealed cyclin E1 mRNA as a candidate. Cyclin E1 is a G1 cyclin and its complex with Cdk2 triggers S phase entry . Cyclin E1 expression is essentially induced by E2F-mediated transcription in late G1, and its protein level is down-regulated by the ubiquitin-proteasome pathway during S phase. We found that depletion of DDX3 impairs cyclin E1 translation, which provides an explanation for DDX3 deficiency-induced G1 arrest. We also observed that cyclin E1 protein level was diminished in hamster cells upon inactivation of temperature-sensitive DDX3 at a non-permissive temperature. DDX3 facilitates cyclin E1 translation probably by resolving potential secondary structures in its 5' UTR, which has a high GC content (~80%). Notably, the Xenopus and Drosophila cyclin E1 mRNAs have long 5' UTRs (~500 nt) , suggesting that DDX3 has an evolutionarily conserved role in regulating cyclin E1 translation. Moreover, phosphorylation of DDX3 by the mitotic Cdk1/cyclin B kinase is thought to cause translational inhibition during mitosis . Therefore, it would be interesting to know whether DDX3 activity is regulated throughout the cell cycle, which in turn could affect the translation of downstream cell cycle factors.
Translational control of cyclin E2 by HCMV miR-US25-1
Human cytomegalovirus (HCMV), a member of the herpesvirus family, encodes at least 11 miRNAs. miR-US25-1 is one of the most highly expressed viral miRNAs during HCMV infection . Interestingly, most miR-US25-1 target genes have been identified as cell cycle regulators, including CCNE2 (cyclin E2) . The miR-US25-1 target sites are usually located within the 5' UTR rather than the 3' UTR of those identified transcripts. miR-US25-1 suppressed the expression of a reporter containing the 5' UTR of cyclin E2, whereas deletion of miR-US25-1 from HCMV up-regulates cyclin E2 expression in the context of viral infection. HCMV infection causes resting cells to re-enter the cell cycle but blocks further progression at the G1/S boundary in order to conduce viral DNA replication. Therefore, perhaps for this purpose, HCMV down-regulates cyclin E2 by expressing miRNAs.
Translational control of cyclin D1 by miRNAs
The miR-17/92 cluster of a ~1 kb encodes seven distinct miRNAs within intron 3 of the C13orf25 gene. The observed inverse correlation between the levels of two cluster members, miR-17-5p/20a and cyclin D1, in human breast tumors led to the identification of miR-17-5p/20a as a translational suppressor of cyclin D1 mRNA via binding to its 3' UTR . Accordingly, expression of these two miRNAs could arrest cell cycle at G1 phase and suppress cell proliferation and tumor colony formation. Interestingly, cyclin D1 can transcriptionally up-regulate miR-17/92 expression via binding to its promoter. Therefore, a possible feedback control loop between cyclin D1 and the miR-17/92 cluster may help cells maintain a desired level of cyclin D1. In addition, cyclin D1 was recently identified as a target of miR-193b in melanoma . Because miRNAs can regulate the expression of a myriad of eukaryotic genes and have been implicated in cancer pathogenesis , future studies will undoubtedly uncover more cell cycle regulators (including cyclins) as targets of miRNAs. Moreover, accumulating evidence indicating that miRNA activities can be modulated by cellular factors . For example, upon induction of differentiation, the RNA-binding protein RBM4 translocates from the nucleus to the cytoplasm and potentiates the suppressive effect of muscle-specific miR-1 on cyclin D1 translation in muscle cells . Further studies will be required to gain more insights into how the regulation of miRNAs affects cell functions and pathogenesis.
Translational control of B-type cyclins by RNA helicases in fission yeast
The DEAD-box RNA helicase Ded1 of the budding yeast has been implicated in the control of translation initiation . Inactivation of Ded1 in S. pombe particularly impairs the translation of G1/S-specific B-type cyclin Cig2 that functions to promote the onset of S phase . Ded1 inactivation also inhibited another B-type but G2/M-specific cyclin, Cdc13, albeit to a lesser extent than Cig2. Another study showed that translation of mRNAs encoding Cdc13 and tyrosine phosphatase Cdc25, which is also a key mitotic regulator, was impaired in the mutants with diminished eIF4A activity . Because the 5' UTR of each of these mRNAs is long or even may contain an uORF(s), their translation thus require RNA helicase activity to facilitate ribosomal scanning or bypass the inhibitory effect of uORFs. These results also suggest that the expression of certain key cell cycle regulators may be tuned at the level of translation.
Translational control of cyclin A by Bruno in Drosophila and Xenopus
A-type cyclins play a role during S phase of mitotic cell cycle. During oogenesis, the meiotic cell cycle is arrested initially at prophase I to permit oocyte differentiation and subsequently at metaphase to complete meiosis. Reduction of cyclin A levels early in meiotic prophase is important to maintain the oocyte in meiosis. The translational inhibitor Bruno suppresses translation of cyclin A mRNA by binding to BREs present in its 3' UTR , but the underlying mechanism is not clear. Moreover, the poly(A) tail of cyclin A mRNA is shortened by the CCR4-containing deadenylase complex during prophase I arrest, which may affect cyclin A mRNA stability or translation [77, 78]. During oocyte maturation, cyclin A1 mRNA resumes translation upon its polyadenylation, which is likely mediated by GLD2 poly(A) polymerase, as well as the loss of Bruno . Therefore, translation suppression of cyclin A1 requires both the timely expression of an RNA-binding protein and polyadenylation control. During organogenesis of Xenopus, the Bruno-like protein BrunoL1 is also involved in translational regulation of cyclin A2, which is important for endodermal cell proliferation . In contrast to Drosophila Bruno, however, Xenopus BrunoL1 activates translation of cyclin A2, although the detailed mechanism remains to be elucidated.
Translational control of cyclin B by cytoplasmic polyadenylation
Cytoplasmic polyadenylation is important for translational control of maternal mRNAs during oogenesis and early embryonic development in many animal species [45, 46]. The Xenopus cyclin B1 mRNA is a paradigm of cytoplasmic polyadenylation-mediated translational control; its translation is suppressed by shortening of its poly(A) tail in arrested oocytes during the onset of meiotic divisions, and polyadenylation and subsequent translation are induced upon hormone stimulation. Such translational control of cyclin B is also observed during oocyte maturation in mammals . Translation of Drosophila maternal cyclin B mRNA is suppressed in the primordial germ cells in order to maintain G2 arrest in the embryonic pole cells . The translational suppressor pumilio binds to the Nanos responsive element in the 3' UTR of cyclin B mRNA and cooperates with Nanos to recruit the CCR4-NOT deadenylase complex . Therefore, mRNA deadenylation also appears to mediate translation regulation of cyclin B1 in the Drosophila germline.
Translational control of CLB3 in budding yeast during meiosis
Meiosis is a specialized cell division event comprising two chromosome segregation phases, namely the separation of homologous chromosomes in meiosis I and of sister chromatids in meiosis II. Accurate Clb3/CDK regulation is essential for chromosome segregation. During the meiotic cell cycle, three B-type cyclin genes, including CLB3, are expressed in meiosis I. However, the activity of Clb3/CDK, which is important for appropriate meiotic chromosome segregation, appears till the onset of meiosis II. Translation of CLB3 is likely suppressed in meiosis I and reactivated in meiosis II . The 5' UTR of CLB3 mRNA is necessary for its timely restricted translation, but the underlying mechanism remains to be explored.
The mTOR/PI3K/Akt pathway is involved in the control of both mRNA translation and cell cycle progression and has long been considered a target for anti-cancer therapy [13, 14, 83, 84]. Rapamycin binds to peptidyl-prolyl cis-trans isomerase FKBPs and to the FKBP-rapamycin binding domain of mTOR and thus prevents mTORC1 complex formation and subsequent signaling . Therefore, certain rapamycin analogs have been approved for anticancer therapy . However, because mTORC2 is much less sensitive to rapamycin , new active-site inhibitors of mTOR have been developed that may completely block the activity of both mTOR-dependent pathways . In addition, because mTOR activity is more effectively suppressed under cell stress conditions such as hypoxia and nutrient deficiency, the use of an AMPK agonist to mimic the effect of energy deprivation also may provide an optional therapeutic strategy . Finally, suppression of cyclin expression can block the cell cycle. Therefore, ERK inhibitors that prevent cyclin D1 expression during mTOR inhibition show a potential for combined therapy by targeting both the mTOR and MAPK pathways .
Because cyclins are central to the control of cell cycle progression, and each acts in a phase-specific manner, cells and embryos must precisely manipulate cyclin levels to promote or suppress the transition between cell cycle phases for different cellular events. Although translational control is not the major mechanism for fluctuation of cyclin levels during the cell cycle, it is important for expression of certain cyclins at specific cell cycle stage, and also provides a means for cells to promptly alter cyclin expression in response to environmental changes. Notably, different translation mechanisms are used to control the expression of different cyclins. Moreover, dysregulation of cyclin expression may cause various diseases involving cell proliferation defects, such as cancer and inflammation. Therefore, a comprehensive understanding of specific mechanisms for the control of cyclin protein expression will provide the basis for developing therapeutic strategy, and perhaps will also potentiate our understanding of stem cell differentiation.
Pyronnet S, Sonenberg N: Cell-cycle-dependent translational control. Curr Opin Genet Dev 2001, 11: 13–18. 10.1016/S0959-437X(00)00150-7
Holcik M, Sonenberg N: Translational control in stress and apoptosis. Nat Rev Mol Cell Biol 2005, 6: 318–327. 10.1038/nrm1618
Johnson DG, Walker CL: Cyclins and cell cycle checkpoints. Annu Rev Pharmacol Toxicol 1999, 39: 295–312. 10.1146/annurev.pharmtox.39.1.295
Hershko A: Roles of ubiquitin-mediated proteolysis in cell cycle control. Curr Opin Cell Biol 1997, 9: 788–799. 10.1016/S0955-0674(97)80079-8
Lania L, Majello B, Napolitano G: Transcriptional control by cell-cycle regulators: a review. J Cell Physiol 1999, 179: 134–141. 10.1002/(SICI)1097-4652(199905)179:2<134::AID-JCP3>3.0.CO;2-O
Jackson RJ, Hellen CU, Pestova TV: The mechanism of eukaryotic translation initiation and principles of its regulation. Nat Rev Mol Cell Biol 2010, 11: 113–127. 10.1038/nrm2838
Pestova T, Lorsch JR, Hellen CUT: The mechanism of translation initiation in eukaryotes. In Translational Control in Biology and Medicine. Volume 48. 3rd edition. Edited by: Mathews MB, Sonenberg N, Hershey JWB. New York: The Cold Spring Harbor Monograph Archive; 2007:87–128.
Stevenson AL, McCarthy JE: Found in translation: another RNA helicase function. Mol Cell 2008, 32: 755–756. 10.1016/j.molcel.2008.12.008
Raught B, Gingras AC: Signaling to Translation Initiation. In Translational Control in Biology and Medicine. Volume 48. 3rd edition. Edited by: Mathews MB, Sonenberg N, Hershey JWB. New York: The Cold Spring Harbor Monograph Archive; 2007:369–400.
Long X, Ortiz-Vega S, Lin Y, Avruch J: Rheb binding to mammalian target of rapamycin (mTOR) is regulated by amino acid sufficiency. J Biol Chem 2005, 280: 23433–23436. 10.1074/jbc.C500169200
Averous J, Fonseca BD, Proud CG: Regulation of cyclin D1 expression by mTORC1 signaling requires eukaryotic initiation factor 4E-binding protein 1. Oncogene 2008, 27: 1106–1113. 10.1038/sj.onc.1210715
Salaun P, Le Breton M, Morales J, Belle R, Boulben S, Mulner-Lorillon O, Cormier P: Signal transduction pathways that contribute to CDK1/cyclin B activation during the first mitotic division in sea urchin embryos. Exp Cell Res 2004, 296: 347–357. 10.1016/j.yexcr.2004.02.013
Tee AR, Blenis J: mTOR, translational control and human disease. Semin Cell Dev Biol 2005, 16: 29–37. 10.1016/j.semcdb.2004.11.005
Silvera D, Formenti SC, Schneider RJ: Translational control in cancer. Nat Rev Cancer 2010, 10: 254–266. 10.1038/nrc2824
Gebauer F, Hentze MW: Molecular mechanisms of translational control. Nat Rev Mol Cell Biol 2004, 5: 827–835. 10.1038/nrm1488
Fingar DC, Richardson CJ, Tee AR, Cheatham L, Tsou C, Blenis J: mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E. Mol Cell Biol 2004, 24: 200–216. 10.1128/MCB.24.1.200-216.2004
Sonenberg N, Gingras AC: The mRNA 5' cap-binding protein eIF4E and control of cell growth. Curr Opin Cell Biol 1998, 10: 268–275. 10.1016/S0955-0674(98)80150-6
Scheper GC, Proud CG: Does phosphorylation of the cap-binding protein eIF4E play a role in translation initiation? Eur J Biochem 2002, 269: 5350–5359. 10.1046/j.1432-1033.2002.03291.x
Bianchini A, Loiarro M, Bielli P, Busa R, Paronetto MP, Loreni F, Geremia R, Sette C: Phosphorylation of eIF4E by MNKs supports protein synthesis, cell cycle progression and proliferation in prostate cancer cells. Carcinogenesis 2008, 29: 2279–2288. 10.1093/carcin/bgn221
Culjkovic B, Topisirovic I, Skrabanek L, Ruiz-Gutierrez M, Borden KL: eIF4E promotes nuclear export of cyclin D1 mRNAs via an element in the 3'UTR. J Cell Biol 2005, 169: 245–256. 10.1083/jcb.200501019
Wek RC, Jiang HY, Anthony TG: Coping with stress: eIF2 kinases and translational control. Biochem Soc Trans 2006, 34: 7–11. 10.1042/BST0340007
Proud CG: eIF2 and the control of cell physiology. Semin Cell Dev Biol 2005, 16: 3–12. 10.1016/j.semcdb.2004.11.004
Wang L, Liu Y, Wu S: The roles of nitric oxide synthase and eIF2alpha kinases in regulation of cell cycle upon UVB-irradiation. Cell Cycle 2010, 9: 38–42. 10.4161/cc.9.1.10268
White-Gilbertson S, Kurtz DT, Voelkel-Johnson C: The role of protein synthesis in cell cycling and cancer. Mol Oncol 2009, 3: 402–408. 10.1016/j.molonc.2009.05.003
Smith EM, Proud CG: cdc2-cyclin B regulates eEF2 kinase activity in a cell cycle- and amino acid-dependent manner. EMBO J 2008, 27: 1005–1016. 10.1038/emboj.2008.39
Hentze MW, Gebauer F, Preiss T: cis-Regulatory Sequences and trans-Acting Factors in Translational Control. In Translational Control in Biology and Medicine. Volume 48. 3rd edition. Edited by: Mathews MB, Sonenberg N, Hershey JWB. New York: The Cold Spring Harbor Monograph Archive; 2007:269–295.
Churbanov A, Rogozin IB, Babenko VN, Ali H, Koonin EV: Evolutionary conservation suggests a regulatory function of AUG triplets in 5'-UTRs of eukaryotic genes. Nucleic Acids Res 2005, 33: 5512–5520. 10.1093/nar/gki847
Hinnebusch AG, Natarajan K: Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress. Eukaryot Cell 2002, 1: 22–32. 10.1128/EC.01.1.22-32.2002
Ivanov IP, Atkins JF, Michael AJ: A profusion of upstream open reading frame mechanisms in polyamine-responsive translational regulation. Nucleic Acids Res 2010, 38: 353–359. 10.1093/nar/gkp1037
Stoneley M, Willis AE: Aberrant regulation of translation initiation in tumorigenesis. Curr Mol Med 2003, 3: 597–603. 10.2174/1566524033479474
Brown CY, Mize GJ, Pineda M, George DL, Morris DR: Role of two upstream open reading frames in the translational control of oncogene mdm2. Oncogene 1999, 18: 5631–5637. 10.1038/sj.onc.1202949
Wethmar K, Begay V, Smink JJ, Zaragoza K, Wiesenthal V, Dorken B, Calkhoven CF, Leutz A: C/EBPbetaDeltauORF mice--a genetic model for uORF-mediated translational control in mammals. Genes Dev 2010, 24: 15–20. 10.1101/gad.557910
Kozak M: An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 1987, 15: 8125–8148. 10.1093/nar/15.20.8125
Allison RS, Mumy ML, Wakefield LM: Translational control elements in the major human transforming growth factor-beta 1 mRNA. Growth Factors 1998, 16: 89–100. 10.3109/08977199809002120
Landers JE, Cassel SL, George DL: Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res 1997, 57: 3562–3568.
Sobczak K, Krzyzosiak WJ: Structural determinants of BRCA1 translational regulation. J Biol Chem 2002, 277: 17349–17358. 10.1074/jbc.M109162200
Parsyan A, Shahbazian D, Martineau Y, Petroulakis E, Alain T, Larsson O, Mathonnet G, Tettweiler G, Hellen CU, Pestova TV, et al.: The helicase protein DHX29 promotes translation initiation, cell proliferation, and tumorigenesis. Proc Natl Acad Sci USA 2009, 106: 22217–22222. 10.1073/pnas.0909773106
Hartman TR, Qian S, Bolinger C, Fernandez S, Schoenberg DR, Boris-Lawrie K: RNA helicase A is necessary for translation of selected messenger RNAs. Nat Struct Mol Biol 2006, 13: 509–516. 10.1038/nsmb1092
Lai MC, Lee YH, Tarn WY: The DEAD-box RNA helicase DDX3 associates with export messenger ribonucleoproteins as well as tip-associated protein and participates in translational control. Mol Biol Cell 2008, 19: 3847–3858. 10.1091/mbc.E07-12-1264
Lai MC, Chang WC, Shieh SY, Tarn WY: DDX3 regulates cell growth through translational control of cyclin E1. Mol Cell Biol 2010, 30: 5444–5453. 10.1128/MCB.00560-10
Doudna JA, Sarnow P: Translation Initiation by Viral Internal Ribosome Entry Sites. In Translational Control in Biology and Medicine. Volume 48. 3rd edition. Edited by: Mathews MB, Sonenberg N, Hershey JWB. New York: The Cold Spring Harbor Monograph Archive; 2007:129–153.
Holcik M: Targeting translation for treatment of cancer--a novel role for IRES? Curr Cancer Drug Targets 2004, 4: 299–311. 10.2174/1568009043333005
Pyronnet S, Pradayrol L, Sonenberg N: A cell cycle-dependent internal ribosome entry site. Mol Cell 2000, 5: 607–616. 10.1016/S1097-2765(00)80240-3
Gilbert WV: Alternative ways to think about cellular internal ribosome entry. J Biol Chem 2010, 285: 29033–29038. 10.1074/jbc.R110.150532
Radford HE, Meijer HA, de Moor CH: Translational control by cytoplasmic polyadenylation in Xenopus oocytes. Biochim Biophys Acta 2008, 1779: 217–229.
Richter JD: CPEB: a life in translation. Trends Biochem Sci 2007, 32: 279–285. 10.1016/j.tibs.2007.04.004
Kim JH, Richter JD: Opposing polymerase-deadenylase activities regulate cytoplasmic polyadenylation. Mol Cell 2006, 24: 173–183. 10.1016/j.molcel.2006.08.016
Minshall N, Reiter MH, Weil D, Standart N: CPEB interacts with an ovary-specific eIF4E and 4E-T in early Xenopus oocytes. J Biol Chem 2007, 282: 37389–37401. 10.1074/jbc.M704629200
Jung MY, Lorenz L, Richter JD: Translational control by neuroguidin, a eukaryotic initiation factor 4E and CPEB binding protein. Mol Cell Biol 2006, 26: 4277–4287. 10.1128/MCB.02470-05
Wilhelm JE, Hilton M, Amos Q, Henzel WJ: Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz. J Cell Biol 2003, 163: 1197–1204. 10.1083/jcb.200309088
Chekulaeva M, Hentze MW, Ephrussi A: Bruno acts as a dual repressor of oskar translation, promoting mRNA oligomerization and formation of silencing particles. Cell 2006, 124: 521–533. 10.1016/j.cell.2006.01.031
Horb LD, Horb ME: BrunoL1 regulates endoderm proliferation through translational enhancement of cyclin A2 mRNA. Dev Biol 2010, 345: 156–169. 10.1016/j.ydbio.2010.07.005
Barreau C, Paillard L, Mereau A, Osborne HB: Mammalian CELF/Bruno-like RNA-binding proteins: molecular characteristics and biological functions. Biochimie 2006, 88: 515–525. 10.1016/j.biochi.2005.10.011
Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004, 116: 281–297. 10.1016/S0092-8674(04)00045-5
Chang TC, Mendell JT: microRNAs in vertebrate physiology and human disease. Annu Rev Genomics Hum Genet 2007, 8: 215–239. 10.1146/annurev.genom.8.080706.092351
Chivukula RR, Mendell JT: Circular reasoning: microRNAs and cell-cycle control. Trends Biochem Sci 2008, 33: 474–481. 10.1016/j.tibs.2008.06.008
Kedde M, van Kouwenhove M, Zwart W, Oude Vrielink JA, Elkon R, Agami R: A Pumilio-induced RNA structure switch in p27–3' UTR controls miR-221 and miR-222 accessibility. Nat Cell Biol 2010, 12: 1014–1020. 10.1038/ncb2105
Vasudevan S, Tong Y, Steitz JA: Switching from repression to activation: microRNAs can up-regulate translation. Science 2007, 318: 1931–1934. 10.1126/science.1149460
Vasudevan S, Tong Y, Steitz JA: Cell-cycle control of microRNA-mediated translation regulation. Cell Cycle 2008, 7: 1545–1549. 10.4161/cc.7.11.6018
Malumbres M, Barbacid M: Cell cycle, CDKs and cancer: a changing paradigm. Nat Rev Cancer 2009, 9: 153–166. 10.1038/nrc2602
Altmann M, Trachsel H: Altered mRNA cap recognition activity of initiation factor 4E in the yeast cell cycle division mutant cdc33. Nucleic Acids Res 1989, 17: 5923–5931. 10.1093/nar/17.15.5923
Hanic-Joyce PJ, Johnston GC, Singer RA: Regulated arrest of cell proliferation mediated by yeast prt1 mutations. Exp Cell Res 1987, 172: 134–145. 10.1016/0014-4827(87)90100-5
Polymenis M, Schmidt EV: Coupling of cell division to cell growth by translational control of the G1 cyclin CLN3 in yeast. Genes Dev 1997, 11: 2522–2531. 10.1101/gad.11.19.2522
Lee CS, Dias AP, Jedrychowski M, Patel AH, Hsu JL, Reed R: Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Nucleic Acids Res 2008, 36: 4708–4718. 10.1093/nar/gkn454
Sekiguchi T, Kurihara Y, Fukumura J: Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin B/cdc2 in vitro. Biochem Biophys Res Commun 2007, 356: 668–673. 10.1016/j.bbrc.2007.03.038
Fannin Rider PJ, Dunn W, Yang E, Liu F: Human cytomegalovirus microRNAs. Curr Top Microbiol Immunol 2008, 325: 21–39. full_text
Grey F, Tirabassi R, Meyers H, Wu G, McWeeney S, Hook L, Nelson JA: A viral microRNA down-regulates multiple cell cycle genes through mRNA 5'UTRs. PLoS Pathog 2010, 6: e1000967. 10.1371/journal.ppat.1000967
Yu Z, Wang C, Wang M, Li Z, Casimiro MC, Liu M, Wu K, Whittle J, Ju X, Hyslop T, et al.: A cyclin D1/microRNA 17/20 regulatory feedback loop in control of breast cancer cell proliferation. J Cell Biol 2008, 182: 509–517. 10.1083/jcb.200801079
Chen J, Feilotter HE, Pare GC, Zhang X, Pemberton JG, Garady C, Lai D, Yang X, Tron VA: MicroRNA-193b represses cell proliferation and regulates cyclin D1 in melanoma. Am J Pathol 2010, 176: 2520–2529. 10.2353/ajpath.2010.091061
Agami R: microRNAs, RNA binding proteins and cancer. Eur J Clin Invest 2010, 40: 370–374. 10.1111/j.1365-2362.2010.02279.x
Steitz JA, Vasudevan S: miRNPs: versatile regulators of gene expression in vertebrate cells. Biochem Soc Trans 2009, 37: 931–935. 10.1042/BST0370931
Lin JC, Tarn WY: RNA-binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 during muscle cell differentiation. J Biol Chem 2009, 284: 34658–34665. 10.1074/jbc.M109.032946
Chuang RY, Weaver PL, Liu Z, Chang TH: Requirement of the DEAD-Box protein ded1p for messenger RNA translation. Science 1997, 275: 1468–1471. 10.1126/science.275.5305.1468
Grallert B, Kearsey SE, Lenhard M, Carlson CR, Nurse P, Boye E, Labib K: A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls. J Cell Sci 2000,113(Pt 8):1447–1458.
Daga RR, Jimenez J: Translational control of the cdc25 cell cycle phosphatase: a molecular mechanism coupling mitosis to cell growth. J Cell Sci 1999,112(Pt 18):3137–3146.
Futcher B: Cyclins in meiosis: lost in translation. Dev Cell 2008, 14: 644–645. 10.1016/j.devcel.2008.04.009
Morris JZ, Hong A, Lilly MA, Lehmann R: twin, a CCR4 homolog, regulates cyclin poly(A) tail length to permit Drosophila oogenesis. Development 2005, 132: 1165–1174. 10.1242/dev.01672
Sugimura I, Lilly MA: Bruno inhibits the expression of mitotic cyclins during the prophase I meiotic arrest of Drosophila oocytes. Dev Cell 2006, 10: 127–135. 10.1016/j.devcel.2005.10.018
Vardy L, Pesin JA, Orr-Weaver TL: Regulation of Cyclin A protein in meiosis and early embryogenesis. Proc Natl Acad Sci USA 2009, 106: 1838–1843. 10.1073/pnas.0813237106
Tay J, Hodgman R, Richter JD: The control of cyclin B1 mRNA translation during mouse oocyte maturation. Dev Biol 2000, 221: 1–9. 10.1006/dbio.2000.9669
Kadyrova LY, Habara Y, Lee TH, Wharton RP: Translational control of maternal Cyclin B mRNA by Nanos in the Drosophila germline. Development 2007, 134: 1519–1527. 10.1242/dev.002212
Carlile TM, Amon A: Meiosis I is established through division-specific translational control of a cyclin. Cell 2008, 133: 280–291. 10.1016/j.cell.2008.02.032
Livingstone M, Atas E, Meller A, Sonenberg N: Mechanisms governing the control of mRNA translation. Phys Biol 2010, 7: 021001. 10.1088/1478-3975/7/2/021001
Sabatini DM: mTOR and cancer: insights into a complex relationship. Nat Rev Cancer 2006, 6: 729–734. 10.1038/nrc1974
Guertin DA, Sabatini DM: An expanding role for mTOR in cancer. Trends Mol Med 2005, 11: 353–361. 10.1016/j.molmed.2005.06.007
Dowling RJ, Topisirovic I, Fonseca BD, Sonenberg N: Dissecting the role of mTOR: lessons from mTOR inhibitors. Biochim Biophys Acta 2010, 1804: 433–439.
Frost P, Shi Y, Hoang B, Gera J, Lichtenstein A: Regulation of D-cyclin translation inhibition in myeloma cells treated with mammalian target of rapamycin inhibitors: rationale for combined treatment with extracellular signal-regulated kinase inhibitors and rapamycin. Mol Cancer Ther 2009, 8: 83–93. 10.1158/1535-7163.MCT-08-0254
We thank Yi-Shiuan Huang (Academia Sinica, Taiwan) for comments on this manuscript. This work was supported by Grant NHRI-EX100-10046NI from the National Health Research Institutes of Taiwan to W.-Y. Tarn and Grant HUA 99-25-7-159 from the National Cheng Kung University of Taiwan to M.-C. Lai.
WYT and MCL contributed equally to manuscript preparation. Both authors read and approved the final manuscript.
Conflict of interests
The authors declare that they have no competing interests.
About this article
Cite this article
Tarn, W., Lai, M. Translational control of cyclins. Cell Div 6, 5 (2011). https://doi.org/10.1186/1747-1028-6-5
- Translation Initiation
- Oocyte Maturation
- mTOR Signaling
- Translational Control
- HCMV Infection