Articles

Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis

The discovery of molecular markers associated with various breast cancer subtypes has greatly improved the treatment and outcome of breast cancer patients. Unfortunately, breast cancer cells acquire resistance...

Authors: Katie R Hagen, Xiangbin Zeng, Mi-Young Lee, Shannon Tucker Kahn, Mary Kathryn Harrison Pitner, Sandra S Zaky, Yuan Liu, Ruth M O’Regan, Xingming Deng and Harold I Saavedra

Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast

The execution of meiotic nuclear divisions in S. cerevisiae is regulated by protein degradation mediated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase. The correct timing of APC/C activity ...

Authors: Grace S Tan, Rebecca Lewandowski, Michael J Mallory, Randy Strich and Katrina F Cooper

To divide or not to divide: revisiting liver regeneration

The liver has a remarkable capacity to regenerate. Even with surgical removal (partial hepatectomy) of 70% of liver mass, the remnant tissue grows to recover the original mass and functions. Liver regeneration...

Authors: Yuichiro Miyaoka and Atsushi Miyajima

Cell cycle-dependent localization of CHK2 at centrosomes during mitosis

Centrosomes function primarily as microtubule-organizing centres and play a crucial role during mitosis by organizing the bipolar spindle. In addition to this function, centrosomes act as reaction centers wher...

Authors: Guillaume Chouinard, Isabelle Clément, Julie Lafontaine, Francis Rodier and Estelle Schmitt

A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis

The yeast Schizosaccharomyces pombe is frequently used as a model for studying the cell cycle. The cells are rod-shaped and divide by medial fission. The process of cell division, or cytokinesis, is controlled by...

Authors: Daniel Schmitter, Paulina Wachowicz, Daniel Sage, Anastasia Chasapi, Ioannis Xenarios, Viesturs Simanis and Michael Unser

Proteolysis of Xenopus Cip-type CDK inhibitor, p16^Xic2, is regulated by PCNA binding and CDK2 phosphorylation

Cell division is positively regulated by cyclin-dependent kinases (CDKs) partnered with cyclins and negatively regulated by CDK inhibitors. In the frog, Xenopus laevis, three types of CDK inhibitors have been des...

Authors: Xi-Ning Zhu, Dong Hyun Kim, Horng-Ru Lin, Varija N Budhavarapu, Herbert B Rosenbaum, Paul R Mueller and P Renee Yew

Rad53 homologue forkhead-associated kinase A (FhkA) and Ca²⁺-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium

During mitosis most nucleolar proteins redistribute to other locales providing an opportunity to study the relationship between nucleolar protein localization and function. Dictyostelium is a model organism for t...

Authors: Andrew Catalano and Danton H O’Day

The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis

Proteins of the BTB-kelch family are known to be involved in multiple biological processes such as migration, cytoskeleton arrangement, regulation of cell morphology, protein ubiquitination and gene expression...

Authors: Sandra Lührig, Susanne Kolb, Nadine Mellies and Jessica Nolte

Ndc80 Loop as a protein-protein interaction motif

Our understanding of the structure and function of kinetochores has advanced dramatically over the past 10 years, yet how the plus end of spindle microtubules interacts with the kinetochore and establishes amp...

Authors: Ngang Heok Tang and Takashi Toda

The role of β-adrenergic receptor signaling in the proliferation of hemangioma-derived endothelial cells

Infantile hemangioma (IH) is a benign vascular neoplasm that arises from the abnormal proliferation of endothelial cells and enhanced angiogenesis. Recently, propranolol has been found to be effective in the m...

Authors: Yi Ji, Siyuan Chen, Kai Li, Xianmin Xiao, Shan Zheng and Ting Xu

Therapeutic targeting of cancer cell cycle using proteasome inhibitors

Proteasomes are multicatalytic protease complexes in the cell, involved in the non-lysosomal recycling of intra-cellular proteins. Proteasomes play a critical role in regulation of cell division in both normal...

Authors: Namrata Rastogi and Durga Prasad Mishra

Mitofusin 1 is degraded at G₂/M phase through ubiquitylation by MARCH5

Mitochondria exhibit a dynamic morphology in cells and their biogenesis and function are integrated with the nuclear cell cycle. In mitotic cells, the filamentous network structure of mitochondria takes on a f...

Authors: Yong-Yea Park and Hyeseong Cho

Identification of new cell size control genes in S. cerevisiae

Cell size homeostasis is a conserved attribute in many eukaryotic species involving a tight regulation between the processes of growth and proliferation. In budding yeast S. cerevisiae, growth to a “critical cell...

Authors: Huzefa Dungrawala, Hui Hua, Jill Wright, Lesley Abraham, Thivakorn Kasemsri, Anthony McDowell, Jessica Stilwell and Brandt L Schneider

Replication of somatic micronuclei in bovine enucleated oocytes

Microcell-mediated chromosome transfer (MMCT) was developed to introduce a low number of chromosomes into a host cell. We have designed a novel technique combining part of MMCT with somatic cell nuclear transf...

Authors: Natalia Canel, Romina Bevacqua, María Inés Hiriart and Daniel Salamone

Emerging players in the initiation of eukaryotic DNA replication

Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase; transition to the pre-initiation complex (pre...

Authors: Zhen Shen and Supriya G Prasanth

Epitope of titin A-band-specific monoclonal antibody Tit1 5 H1.1 is highly conserved in several Fn3 domains of the titin molecule. Centriole staining in human, mouse and zebrafish cells

Previously we have reported on the development of a new mouse anti-titin monoclonal antibody, named MAb Titl 5 H1.1, using the synthetic peptide N-AVNKYGIGEPLESDSVVAK-C which corresponds to an amino acid seque...

Authors: Aavo-Valdur Mikelsaar, Alar Sünter, Ruth Mikelsaar, Peeter Toomik, Anu Kõiveer, Imre Mikelsaar and Erkki Juronen

Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells

Buddleja davidii is widely distributed in the southwestern region of China. We have undertaken a systematic analysis of B. davidii as a Chinese traditional medicine with anticancer activity by isolating natural ...

Authors: Jian Wu, Wenshi Yi, Linhong Jin, Deyu Hu and Baoan Song

Transient defects of mitotic spindle geometry and chromosome segregation errors

Assembly of a bipolar mitotic spindle is essential to ensure accurate chromosome segregation and prevent aneuploidy, and severe mitotic spindle defects are typically associated with cell death. Recent studies ...

Authors: William T Silkworth and Daniela Cimini

The ubiquitin-proteasome system in glioma cell cycle control

A major determinant of cell fate is regulation of cell cycle. Tight regulation of this process is lost during the course of development and progression of various tumors. The ubiquitin-proteasome system (UPS) ...

Authors: Panagiotis J Vlachostergios, Ioannis A Voutsadakis and Christos N Papandreou

New insights into subcomplex assembly and modifications of centrosomal proteins

This review provides a brief overview of the recent work on centrosome proteomics, protein complex identification and functional characterization with an emphasis on the literature of the last three years. Pro...

Authors: Karin Habermann and Bodo MH Lange

Cytotoxicity, anti-angiogenic, apoptotic effects and transcript profiling of a naturally occurring naphthyl butenone, guieranone A

Malignant diseases are responsible of approximately 13% of all deaths each year in the world. Natural products represent a valuable source for the development of novel anticancer drugs. The present study was a...

Authors: Victor Kuete, Tolga Eichhorn, Benjamin Wiench, Benjamin Krusche and Thomas Efferth

Retraction Note: Role of senescence and mitotic catastrophe in cancer therapy

Authors: Richa Singh, Jasmine George and Yogeshwer Shukla

Differential requirement of cyclin-dependent kinase 2 for oligodendrocyte progenitor cell proliferation and differentiation

Cyclin-dependent kinases (Cdks) and their cyclin regulatory subunits control cell growth and division. Cdk2-cyclin E complexes, phosphorylating the retinoblastoma protein, drive cells through the G1/S transiti...

Authors: Céline Caillava and Anne Baron-Van Evercooren

A conserved histone deacetylase with a role in the regulation of cytokinesis in Schizosaccharomyces pombe

In Schizosaccharomyces pombe the SET domain protein, Set3p - together with its interacting partners, Snt1p, and Hif2p - form a complex that aids in preventing cell division failure upon mild cytokinetic stress. I...

Authors: Charnpal Grewal, Jack Hickmott, Stefan Rentas and Jim Karagiannis

Cyclin K goes with Cdk12 and Cdk13

The cyclin-dependent kinases (Cdks) regulate many cellular processes, including the cell cycle, neuronal development, transcription, and posttranscriptional processing. To perform their functions, Cdks bind to...

Authors: Jiri Kohoutek and Dalibor Blazek

Complex effects of flavopiridol on the expression of primary response genes

The Positive Transcription Elongation Factor b (P-TEFb) is a complex of Cyclin Dependent Kinase 9 (CDK9) with either cyclins T1, T2 or K. The complex phosphorylates the C-Terminal Domain of RNA polymerase II (...

Authors: Havva Keskin, Judit Garriga, Daphne Georlette and Xavier Graña

The retinoblastoma family of proteins and their regulatory functions in the mammalian cell division cycle

The retinoblastoma (RB) family of proteins are found in organisms as distantly related as humans, plants, and insects. These proteins play a key role in regulating advancement of the cell division cycle from t...

Authors: Shauna A Henley and Frederick A Dick

lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2

Ubiquitin-dependent protein degradation is a critical step in key cell cycle events, such as metaphase-anaphase transition and mitotic exit. The anaphase promoting complex/cyclosome (APC/C) plays a pivotal rol...

Authors: Olga Nagy, Margit Pál, Andor Udvardy, Christine AM Shirras, Imre Boros, Alan D Shirras and Péter Deák

Characterization of the antiproliferative activity of Xylopia aethiopica

Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells.

Authors: Aphrodite T Choumessi, Mathieu Danel, Stefan Chassaing, Isabelle Truchet, Véronique B Penlap, Anatole Constant Pieme, Tazoacha Asonganyi, Bernard Ducommun and Annie Valette

Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1

The inorganic phosphate (P_i) transporter, PiT1 (SLC20A1), is ubiquitously expressed in mammalian cells. It has previously been shown that down-regulation of PiT1 severely impaired the proliferation of two transfo...

Authors: Kristina Byskov, Nina Jensen, Iben Boutrup Kongsfelt, Maria Wielsøe, Lasse Ebdrup Pedersen, Christa Haldrup and Lene Pedersen

Two sides of the Myc-induced DNA damage response: from tumor suppression to tumor maintenance

Activation of oncogenes is generally associated with the induction of DNA damage response (DDR) signaling, which acts as a barrier to tumor progression. In this review we will present an overview of the DDR as...

Authors: Stefano Campaner and Bruno Amati

Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle

The clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during the cellular division is a crucial biological process controlled by the DNA methyltransferase Dnmt1, mainly. To...

Authors: Eric Hervouet, Arulraj Nadaradjane, Marine Gueguen, François M Vallette and Pierre-François Cartron

APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage

The spindle assembly checkpoint (SAC) inhibits anaphase progression in the presence of insufficient kinetochore-microtubule attachments, but cells can eventually override mitotic arrest by a process known as m...

Authors: Kazuhiro Toda, Kayoko Naito, Satoru Mase, Masaru Ueno, Masahiro Uritani, Ayumu Yamamoto and Takashi Ushimaru

Polo-like kinase 1 (Plk1): an Unexpected Player in DNA Replication

Regulation of cell cycle progression is important for the maintenance of genome integrity, and Polo-like kinases (Plks) have been identified as key regulators of this process. It is well established that Polo-...

Authors: Bing Song, X Shawn Liu and Xiaoqi Liu

Bypass of cell cycle arrest induced by transient DNMT1 post-transcriptional silencing triggers aneuploidy in human cells

Aneuploidy has been acknowledged as a major source of genomic instability in cancer, and it is often considered the result of chromosome segregation errors including those caused by defects in genes controllin...

Authors: Viviana Barra, Tiziana Schillaci, Laura Lentini, Giuseppe Costa and Aldo Di Leonardo

The yeast mitogen-activated protein kinase Slt2 is involved in the cellular response to genotoxic stress

The maintenance of genomic integrity is essential for cell viability. Complex signalling pathways (DNA integrity checkpoints) mediate the response to genotoxic stresses. Identifying new functions involved in t...

Authors: María Soriano-Carot, M Carmen Bañó and J Carlos Igual

Phosphorylation of Cdc5 regulates its accumulation

Cdc5 (polo kinase/Plk1) is a highly conserved key regulator of the S. cerevisiae cell cycle from S-phase until cytokinesis. However, much of the regulatory mechanisms that govern Cdc5 remain to be determined. Cdc...

Authors: Kobi J Simpson-Lavy and Michael Brandeis

Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy

Multicellular tumor spheroids are models of increasing interest for cancer and cell biology studies. They allow considering cellular interactions in exploring cell cycle and cell division mechanisms. However, ...

Authors: Corinne Lorenzo, Céline Frongia, Raphaël Jorand, Jérôme Fehrenbach, Pierre Weiss, Amina Maandhui, Guillaume Gay, Bernard Ducommun and Valérie Lobjois

Novel insights into maintaining genomic integrity: Wee1 regulating Mus81/Eme1

Maintenance of genomic integrity is essential for cell survival. Specifically, during DNA replication cells use a complex network of mechanisms that prevents genomic instability. Recently, we and others identi...

Authors: Yusé Martín, Raquel Domínguez-Kelly and Raimundo Freire

Proliferation/Quiescence: When to start? Where to stop? What to stock?

The cell cycle is a tightly controlled series of events that ultimately lead to cell division. The literature deciphering the molecular processes involved in regulating the consecutive cell cycle steps is colo...

Authors: Bertrand Daignan-Fornier and Isabelle Sagot

Crosstalk between NDR kinase pathways coordinates cell cycle dependent actin rearrangements

Regulation of cytoskeletal remodeling is essential for cell cycle transitions. In fission yeast two NDR kinase signaling cascades, MOR and SIN, regulate the actin cytoskeleton to promote polarized growth durin...

Authors: Sneha Gupta and Dannel McCollum

Nek family of kinases in cell cycle, checkpoint control and cancer

Early studies in lower Eukaryotes have defined a role for the members of the NimA related kinase (Nek) family of protein kinases in cell cycle control. Expansion of the Nek family throughout evolution has been...

Authors: Larissa Moniz, Previn Dutt, Nasir Haider and Vuk Stambolic

Mechanism of the chromosome-induced polar body extrusion in mouse eggs

An oocyte undergoes two rounds of asymmetric division to generate a haploid gamete and two small polar bodies designed for apoptosis. Chromosomes play important roles in specifying the asymmetric meiotic divis...

Authors: Qiong Wang, Catherine Racowsky and Manqi Deng

Meiotic control of the APC/C: similarities & differences from mitosis

The anaphase promoting complex is a highly conserved E3 ligase complex that mediates the destruction of key regulatory proteins during both mitotic and meiotic divisions. In order to maintain ploidy, this dest...

Authors: Katrina F Cooper and Randy Strich

Human linker histones: interplay between phosphorylation and O-β-GlcNAc to mediate chromatin structural modifications

Eukaryotic chromatin is a combination of DNA and histone proteins. It is established fact that epigenetic mechanisms are associated with DNA and histones. Initial studies emphasize on core histones association...

Authors: Waqar Ahmad, Khadija Shabbiri, Noreen Nazar, Shazia Nazar, Saba Qaiser and Mirza Abid Shabbir Mughal

Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines

Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma ve...

Authors: Xiaoqiang Xu, Xiuhong Gao, Linhong Jin, Pinaki S Bhadury, Kai Yuan, Deyu Hu, Baoan Song and Song Yang

Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast

Histone post-translational modifications are critical for gene expression and cell viability. A broad spectrum of histone lysine residues have been identified in yeast that are targeted by a variety of modifyi...

Authors: Azharul Islam, Emma L Turner, Johannes Menzel, Mackenzie E Malo and Troy AA Harkness

Regulation of asymmetric cell division in the epidermis

For proper tissue morphogenesis, cell divisions and cell fate decisions must be tightly and coordinately regulated. One elegant way to accomplish this is to couple them with asymmetric cell divisions. Progenit...

Authors: Samriddha Ray and Terry Lechler

New clues to understand how CENP-A maintains centromere identity

The centromere is a specialized chromosomal region that directs the formation of the kinetochore, a huge protein assembly that acts as the attachment site for spindle microtubules and carries out chromosome mo...

Authors: Patricia Sánchez and Ana Losada

Proliferation/quiescence: the controversial "aller-retour"

The vast majority of cells, from prokaryotes up to vertebrate organisms, spend most of their time in quiescence, a state defined as a temporary and reversible absence of proliferation. Establishing the quiesce...

Authors: Bertrand Daignan-Fornier and Isabelle Sagot