Articles

Mutations in the PCNA-binding site of CDKN1C inhibit cell proliferation by impairing the entry into S phase

CDKN1C (also known as P57 ^kip2 ) is a cyclin-dependent kinase inhibitor that functions as a negative regulator of cell proliferation through G1 ...

Authors: Kleiton S Borges, Valerie A Arboleda and Eric Vilain

Cyclin E2 is the predominant E-cyclin associated with NPAT in breast cancer cells

The cyclin E oncogene activates CDK2 to drive cells from G₁ to S phase of the cell cycle to commence DNA replication. It coordinates essential cellular functions with the cell cycle including histone biogenesis, ...

Authors: Samuel Rogers, Brian S Gloss, Christine S Lee, Claudio Marcelo Sergio, Marcel E Dinger, Elizabeth A Musgrove, Andrew Burgess and Catherine Elizabeth Caldon

Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells

Heterochromatin protein 1 (HP1) is important in the establishment, propagation, and maintenance of constitutive heterochromatin, especially at the pericentromeric region. HP1 might participate in recruiting an...

Authors: Rodrigo González-Barrios, Ernesto Soto-Reyes, Ricardo Quiroz-Baez, Eunice Fabián-Morales, José Díaz-Chávez, Victor del Castillo, Julia Mendoza, Alejandro López-Saavedra, Clementina Castro and Luis A Herrera

Polar electrostatic forces drive poleward chromosome motions

Recent experiments revealing nanoscale electrostatic force generation at kinetochores for chromosome motions have prompted models for interactions between positively charged molecules in kinetochores and negat...

Authors: Lucian John Gagliardi and Daniel H Shain

The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence

The S-phase checkpoint aims to prevent cells from generation of extensive single-stranded DNA that predisposes to genome instability. The S. cerevisiae complex Tof1/Csm3/Mrc1 acts to restrain the replicative MCM ...

Authors: Sonya Dimitrova Uzunova, Alexander Stefanov Zarkov, Anna Marianova Ivanova, Stoyno Stefanov Stoynov and Marina Nedelcheva Nedelcheva-Veleva

Differential expression of centrosome regulators in Her2+ breast cancer cells versus non-tumorigenic MCF10A cells

Centrosome amplification (CA) amongst particular breast cancer subtypes (Her2+ subtype) is associated with genomic instability and aggressive tumor phenotypes. However, changes in signaling pathways associated...

Authors: Mi-Young Lee, Mihaela Marina, Jamie L King and Harold I Saavedra

Smurf2 E3 ubiquitin ligase modulates proliferation and invasiveness of breast cancer cells in a CNKSR2 dependent manner

Smurf2 is a member of the HECT family of E3 ubiquitin ligases that play important roles in determining the competence of cells to respond to TGF- β/BMP signaling pathway. However, besides TGF-β/BMP pathway, Sm...

Authors: Diana David, Sankar Jagadeeshan, Ramkumar Hariharan, Asha Sivakumari Nair and Radhakrishna Madhavan Pillai

The DNA repair component Metnase regulates Chk1 stability

Chk1 both arrests replication forks and enhances repair of DNA damage by phosphorylation of downstream effectors. Metnase (also termed SETMAR) is a SET histone methylase and transposase nuclease protein that p...

Authors: Elizabeth A Williamson, Yuehan Wu, Sudha Singh, Michael Byrne, Justin Wray, Suk-Hee Lee, Jac A Nickoloff and Robert Hromas

The COP9 signalosome subunit 6 (CSN6): a potential oncogene

CSN6 is one subunit of the constitutive photomorphogenesis 9 (COP9) signalosome (CSN), which is an evolutionarily conserved multiprotein complex found in plants and animals and originally described as a repres...

Authors: Shang-Nuan Zhang, Dong-Sheng Pei and Jun-Nian Zheng

Defining a new vision for the retinoblastoma gene: report from the 3rd International Rb Meeting

The retinoblastoma tumor suppressor (Rb) pathway is mutated in most, if not all human tumors. In the G0/G1 phase, Rb and its family members p107 and p130 inhibit the E2F family of transcription factors. In res...

Authors: Seth M Rubin and Julien Sage

Cohesin loading factor Nipbl localizes to chromosome axes during mammalian meiotic prophase

Sister chromatid cohesion mediated by the cohesin complex is essential for accurate chromosome segregation during mitosis and meiosis. Loading of cohesin onto chromosomes is dependent on another protein comple...

Authors: Katarzyna Kuleszewicz, Xiangwei Fu and Nobuaki R Kudo

A pool of peptides extracted from wheat bud chromatin inhibits tumor cell growth by causing defective DNA synthesis

We previously reported that a pool of low molecular weight peptides can be extracted by alkali treatment of DNA preparations obtained from prokaryotic and eukaryotic cells after intensive deproteinization. Thi...

Authors: Loretta Mancinelli, Teresa Secca, Paula M De Angelis, Francesco Mancini, Matteo Marchesini, Cristiano Marinelli, Lanfranco Barberini and Francesco Grignani

Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis

The discovery of molecular markers associated with various breast cancer subtypes has greatly improved the treatment and outcome of breast cancer patients. Unfortunately, breast cancer cells acquire resistance...

Authors: Katie R Hagen, Xiangbin Zeng, Mi-Young Lee, Shannon Tucker Kahn, Mary Kathryn Harrison Pitner, Sandra S Zaky, Yuan Liu, Ruth M O’Regan, Xingming Deng and Harold I Saavedra

Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast

The execution of meiotic nuclear divisions in S. cerevisiae is regulated by protein degradation mediated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase. The correct timing of APC/C activity ...

Authors: Grace S Tan, Rebecca Lewandowski, Michael J Mallory, Randy Strich and Katrina F Cooper

To divide or not to divide: revisiting liver regeneration

The liver has a remarkable capacity to regenerate. Even with surgical removal (partial hepatectomy) of 70% of liver mass, the remnant tissue grows to recover the original mass and functions. Liver regeneration...

Authors: Yuichiro Miyaoka and Atsushi Miyajima

Cell cycle-dependent localization of CHK2 at centrosomes during mitosis

Centrosomes function primarily as microtubule-organizing centres and play a crucial role during mitosis by organizing the bipolar spindle. In addition to this function, centrosomes act as reaction centers wher...

Authors: Guillaume Chouinard, Isabelle Clément, Julie Lafontaine, Francis Rodier and Estelle Schmitt

A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis

The yeast Schizosaccharomyces pombe is frequently used as a model for studying the cell cycle. The cells are rod-shaped and divide by medial fission. The process of cell division, or cytokinesis, is controlled by...

Authors: Daniel Schmitter, Paulina Wachowicz, Daniel Sage, Anastasia Chasapi, Ioannis Xenarios, Viesturs Simanis and Michael Unser

Proteolysis of Xenopus Cip-type CDK inhibitor, p16^Xic2, is regulated by PCNA binding and CDK2 phosphorylation

Cell division is positively regulated by cyclin-dependent kinases (CDKs) partnered with cyclins and negatively regulated by CDK inhibitors. In the frog, Xenopus laevis, three types of CDK inhibitors have been des...

Authors: Xi-Ning Zhu, Dong Hyun Kim, Horng-Ru Lin, Varija N Budhavarapu, Herbert B Rosenbaum, Paul R Mueller and P Renee Yew

Rad53 homologue forkhead-associated kinase A (FhkA) and Ca²⁺-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium

During mitosis most nucleolar proteins redistribute to other locales providing an opportunity to study the relationship between nucleolar protein localization and function. Dictyostelium is a model organism for t...

Authors: Andrew Catalano and Danton H O’Day

The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis

Proteins of the BTB-kelch family are known to be involved in multiple biological processes such as migration, cytoskeleton arrangement, regulation of cell morphology, protein ubiquitination and gene expression...

Authors: Sandra Lührig, Susanne Kolb, Nadine Mellies and Jessica Nolte

Ndc80 Loop as a protein-protein interaction motif

Our understanding of the structure and function of kinetochores has advanced dramatically over the past 10 years, yet how the plus end of spindle microtubules interacts with the kinetochore and establishes amp...

Authors: Ngang Heok Tang and Takashi Toda

The role of β-adrenergic receptor signaling in the proliferation of hemangioma-derived endothelial cells

Infantile hemangioma (IH) is a benign vascular neoplasm that arises from the abnormal proliferation of endothelial cells and enhanced angiogenesis. Recently, propranolol has been found to be effective in the m...

Authors: Yi Ji, Siyuan Chen, Kai Li, Xianmin Xiao, Shan Zheng and Ting Xu

Therapeutic targeting of cancer cell cycle using proteasome inhibitors

Proteasomes are multicatalytic protease complexes in the cell, involved in the non-lysosomal recycling of intra-cellular proteins. Proteasomes play a critical role in regulation of cell division in both normal...

Authors: Namrata Rastogi and Durga Prasad Mishra

Mitofusin 1 is degraded at G₂/M phase through ubiquitylation by MARCH5

Mitochondria exhibit a dynamic morphology in cells and their biogenesis and function are integrated with the nuclear cell cycle. In mitotic cells, the filamentous network structure of mitochondria takes on a f...

Authors: Yong-Yea Park and Hyeseong Cho

Identification of new cell size control genes in S. cerevisiae

Cell size homeostasis is a conserved attribute in many eukaryotic species involving a tight regulation between the processes of growth and proliferation. In budding yeast S. cerevisiae, growth to a “critical cell...

Authors: Huzefa Dungrawala, Hui Hua, Jill Wright, Lesley Abraham, Thivakorn Kasemsri, Anthony McDowell, Jessica Stilwell and Brandt L Schneider

Replication of somatic micronuclei in bovine enucleated oocytes

Microcell-mediated chromosome transfer (MMCT) was developed to introduce a low number of chromosomes into a host cell. We have designed a novel technique combining part of MMCT with somatic cell nuclear transf...

Authors: Natalia Canel, Romina Bevacqua, María Inés Hiriart and Daniel Salamone

Emerging players in the initiation of eukaryotic DNA replication

Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase; transition to the pre-initiation complex (pre...

Authors: Zhen Shen and Supriya G Prasanth

Epitope of titin A-band-specific monoclonal antibody Tit1 5 H1.1 is highly conserved in several Fn3 domains of the titin molecule. Centriole staining in human, mouse and zebrafish cells

Previously we have reported on the development of a new mouse anti-titin monoclonal antibody, named MAb Titl 5 H1.1, using the synthetic peptide N-AVNKYGIGEPLESDSVVAK-C which corresponds to an amino acid seque...

Authors: Aavo-Valdur Mikelsaar, Alar Sünter, Ruth Mikelsaar, Peeter Toomik, Anu Kõiveer, Imre Mikelsaar and Erkki Juronen

Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells

Buddleja davidii is widely distributed in the southwestern region of China. We have undertaken a systematic analysis of B. davidii as a Chinese traditional medicine with anticancer activity by isolating natural ...

Authors: Jian Wu, Wenshi Yi, Linhong Jin, Deyu Hu and Baoan Song

Transient defects of mitotic spindle geometry and chromosome segregation errors

Assembly of a bipolar mitotic spindle is essential to ensure accurate chromosome segregation and prevent aneuploidy, and severe mitotic spindle defects are typically associated with cell death. Recent studies ...

Authors: William T Silkworth and Daniela Cimini

The ubiquitin-proteasome system in glioma cell cycle control

A major determinant of cell fate is regulation of cell cycle. Tight regulation of this process is lost during the course of development and progression of various tumors. The ubiquitin-proteasome system (UPS) ...

Authors: Panagiotis J Vlachostergios, Ioannis A Voutsadakis and Christos N Papandreou

New insights into subcomplex assembly and modifications of centrosomal proteins

This review provides a brief overview of the recent work on centrosome proteomics, protein complex identification and functional characterization with an emphasis on the literature of the last three years. Pro...

Authors: Karin Habermann and Bodo MH Lange

Cytotoxicity, anti-angiogenic, apoptotic effects and transcript profiling of a naturally occurring naphthyl butenone, guieranone A

Malignant diseases are responsible of approximately 13% of all deaths each year in the world. Natural products represent a valuable source for the development of novel anticancer drugs. The present study was a...

Authors: Victor Kuete, Tolga Eichhorn, Benjamin Wiench, Benjamin Krusche and Thomas Efferth

Retraction Note: Role of senescence and mitotic catastrophe in cancer therapy

Authors: Richa Singh, Jasmine George and Yogeshwer Shukla

Differential requirement of cyclin-dependent kinase 2 for oligodendrocyte progenitor cell proliferation and differentiation

Cyclin-dependent kinases (Cdks) and their cyclin regulatory subunits control cell growth and division. Cdk2-cyclin E complexes, phosphorylating the retinoblastoma protein, drive cells through the G1/S transiti...

Authors: Céline Caillava and Anne Baron-Van Evercooren

A conserved histone deacetylase with a role in the regulation of cytokinesis in Schizosaccharomyces pombe

In Schizosaccharomyces pombe the SET domain protein, Set3p - together with its interacting partners, Snt1p, and Hif2p - form a complex that aids in preventing cell division failure upon mild cytokinetic stress. I...

Authors: Charnpal Grewal, Jack Hickmott, Stefan Rentas and Jim Karagiannis

Cyclin K goes with Cdk12 and Cdk13

The cyclin-dependent kinases (Cdks) regulate many cellular processes, including the cell cycle, neuronal development, transcription, and posttranscriptional processing. To perform their functions, Cdks bind to...

Authors: Jiri Kohoutek and Dalibor Blazek

Complex effects of flavopiridol on the expression of primary response genes

The Positive Transcription Elongation Factor b (P-TEFb) is a complex of Cyclin Dependent Kinase 9 (CDK9) with either cyclins T1, T2 or K. The complex phosphorylates the C-Terminal Domain of RNA polymerase II (...

Authors: Havva Keskin, Judit Garriga, Daphne Georlette and Xavier Graña

The retinoblastoma family of proteins and their regulatory functions in the mammalian cell division cycle

The retinoblastoma (RB) family of proteins are found in organisms as distantly related as humans, plants, and insects. These proteins play a key role in regulating advancement of the cell division cycle from t...

Authors: Shauna A Henley and Frederick A Dick

lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2

Ubiquitin-dependent protein degradation is a critical step in key cell cycle events, such as metaphase-anaphase transition and mitotic exit. The anaphase promoting complex/cyclosome (APC/C) plays a pivotal rol...

Authors: Olga Nagy, Margit Pál, Andor Udvardy, Christine AM Shirras, Imre Boros, Alan D Shirras and Péter Deák

Characterization of the antiproliferative activity of Xylopia aethiopica

Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells.

Authors: Aphrodite T Choumessi, Mathieu Danel, Stefan Chassaing, Isabelle Truchet, Véronique B Penlap, Anatole Constant Pieme, Tazoacha Asonganyi, Bernard Ducommun and Annie Valette

Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1

The inorganic phosphate (P_i) transporter, PiT1 (SLC20A1), is ubiquitously expressed in mammalian cells. It has previously been shown that down-regulation of PiT1 severely impaired the proliferation of two transfo...

Authors: Kristina Byskov, Nina Jensen, Iben Boutrup Kongsfelt, Maria Wielsøe, Lasse Ebdrup Pedersen, Christa Haldrup and Lene Pedersen

Two sides of the Myc-induced DNA damage response: from tumor suppression to tumor maintenance

Activation of oncogenes is generally associated with the induction of DNA damage response (DDR) signaling, which acts as a barrier to tumor progression. In this review we will present an overview of the DDR as...

Authors: Stefano Campaner and Bruno Amati

Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle

The clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during the cellular division is a crucial biological process controlled by the DNA methyltransferase Dnmt1, mainly. To...

Authors: Eric Hervouet, Arulraj Nadaradjane, Marine Gueguen, François M Vallette and Pierre-François Cartron

APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage

The spindle assembly checkpoint (SAC) inhibits anaphase progression in the presence of insufficient kinetochore-microtubule attachments, but cells can eventually override mitotic arrest by a process known as m...

Authors: Kazuhiro Toda, Kayoko Naito, Satoru Mase, Masaru Ueno, Masahiro Uritani, Ayumu Yamamoto and Takashi Ushimaru

Polo-like kinase 1 (Plk1): an Unexpected Player in DNA Replication

Regulation of cell cycle progression is important for the maintenance of genome integrity, and Polo-like kinases (Plks) have been identified as key regulators of this process. It is well established that Polo-...

Authors: Bing Song, X Shawn Liu and Xiaoqi Liu

Bypass of cell cycle arrest induced by transient DNMT1 post-transcriptional silencing triggers aneuploidy in human cells

Aneuploidy has been acknowledged as a major source of genomic instability in cancer, and it is often considered the result of chromosome segregation errors including those caused by defects in genes controllin...

Authors: Viviana Barra, Tiziana Schillaci, Laura Lentini, Giuseppe Costa and Aldo Di Leonardo

The yeast mitogen-activated protein kinase Slt2 is involved in the cellular response to genotoxic stress

The maintenance of genomic integrity is essential for cell viability. Complex signalling pathways (DNA integrity checkpoints) mediate the response to genotoxic stresses. Identifying new functions involved in t...

Authors: María Soriano-Carot, M Carmen Bañó and J Carlos Igual

Phosphorylation of Cdc5 regulates its accumulation

Cdc5 (polo kinase/Plk1) is a highly conserved key regulator of the S. cerevisiae cell cycle from S-phase until cytokinesis. However, much of the regulatory mechanisms that govern Cdc5 remain to be determined. Cdc...

Authors: Kobi J Simpson-Lavy and Michael Brandeis

Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy

Multicellular tumor spheroids are models of increasing interest for cancer and cell biology studies. They allow considering cellular interactions in exploring cell cycle and cell division mechanisms. However, ...

Authors: Corinne Lorenzo, Céline Frongia, Raphaël Jorand, Jérôme Fehrenbach, Pierre Weiss, Amina Maandhui, Guillaume Gay, Bernard Ducommun and Valérie Lobjois